Evaluation of procedures for isolation of nontuberculous mycobacteria from soil and water.
نویسندگان
چکیده
Six methods of decontamination each for the isolation of mycobacteria from soil and water were compared. On the basis of the results obtained, three of the six methods for soil and two of the six methods for water were further evaluated. For both soil and water samples, the method using 3% sodium lauryl sulfate in combination with 1% NaOH yielded more positives than the other methods.
منابع مشابه
Optimized Method for Isolation of Nontuberculous Mycobacteria from Hospital Aquatic Sources
Background: Aquatic ecosystems are an important source of nontuberculous mycobacteria (NTM) that can cause different diseases in human. Since culture of mycobacteria needs long-term incubation, fast-growing microorganisms and contaminants in the environment usually prevents the isolation of mycobacteria. Here, we compare different treatment protocols and describe a method that increases the rec...
متن کاملIdentification of Nontuberculous Mycobacteria Species Isolated from Water Samples Using Phenotypic and Molecular Methods and Determination of their Antibiotic Resistance Patterns by E- Test Method, in Isfahan, Iran
Introduction Many studies have shown epidemiological links between strains isolated in tap water, and those isolated from patients. Molecular methods linked to PCR are more reliable and faster for identification of non- tuberculous mycobacteria(NTM). In this study molecular methods were used for identification and typing of NTM. Materials and Methods Five hundred ml of 85 water ...
متن کاملThe role of nontuberculous mycobacteria in cutaneous infections of diabetic patients: a literature review
Background: Diabetes is an important metabolic disease with myriad manifestations and complications, affecting many people. Cutaneous infections impact numerous diabetic patients. Among different bacterial infections in diabetic patients, the infections caused by mycobacteria other than Mycobacterium tuberculosis (MOTT) eventuate in complications owing to the paucity of accurate detection...
متن کاملThe Comparison of Biochemical and Sequencing 16S rDNA Gene Methods to Identify Nontuberculous Mycobacteria
The identification of Mycobacteria in the species level has great medical importance. Biochemical tests are laborious and time-consuming, so new techniques could be used to identify the species. This research aimed to the comparison of biochemical and sequencing 16S rDNA gene methods to identify nontuberculous Mycobacteria in patients suspected to tuberculosis in Golestan province which is the ...
متن کاملIsolation and identification of nontuberculous mycobacteria from water and soil in central India.
Nontuberculous mycobacteria (NTM), important organisms in the Genus Mycobacterium and commonly present in the environment, are known to cause disseminated disease in AIDS patients. In this study, NTM were isolated from environment (soil and water) of the AIDS patients with disseminated NTM disease to know the prevalence of environmental NTM species and their correlation with clinical isolates f...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied and environmental microbiology
دوره 60 3 شماره
صفحات -
تاریخ انتشار 1994